Document Details
Document Type |
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Thesis |
Document Title |
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METAGENOMIC ANALYSIS OF CALOTROPIS PROCERA’S RHIZOSPHERE MICROBIOME التحليل الميتاجينومي لميكروبيوم الريزوسفير لنبات العشار |
Subject |
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faculty of science |
Document Language |
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Arabic |
Abstract |
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The microbial community of complex associations of plants is vital for the health of the plant. plants can form the microbiome of their rhizosphere as the new advances research in plant-microbe interaction revealed, as demonstrated the fact that different species of plant, when grown on the same soil, can host-specific microbial communities. The microbiome of Calotropis procera rhizosphere was studied. Deep sequencing was carried out for the V3-V4 regions (~400 bp) of bacterial 16S rRNA. Sequence tags were assigned to 300 OTUs (operational taxonomic units) across samples with at least 97% similarity. The results indicated that the most common phyla are Acidobacteria (two genera), Actinobacteria (fourteen genera), Bacteroidetes (one genus), Proteobacteria (6 genera) and Firmicutes (nine genera). Highly abundant genera included Ammoniphilus, Bacillus, Iamia, Microvirga, Nocardia, Streptomyces, Defluviicoccus, Mycobacterium, Nocardioides, Sphingomonas, Bryobacter, Ammoniphilus, Stenotrophobacter, Arthrobacter, Actinomadura, Marmoricola. At the species level, High abundance of Nocardia cyriacigeorgica, Rhodospirillales bacterium WX36, and Arthrobacter crystallopoietes in Calotropis procera. These results indicate that the rhizosphere of calotropis procera have a lot of bacterial species that help it to overcome the abiotic stress. The very important issues in this study the calotropis procera rhizosphere is very rich with un-classified species which not found in rhizosphere free plants. |
Supervisor |
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Dr. Ahmed Mohamed Mohamed Emara |
Thesis Type |
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Master Thesis |
Publishing Year |
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1442 AH
2020 AD |
Co-Supervisor |
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Dr. Nour Eldin Omar Gaddallah |
Added Date |
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Tuesday, August 25, 2020 |
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Researchers
محمد عبد القدوس حق نذر | Haq Nazar, Mohammad Abdul Qudus | Researcher | Master | |
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