Main Page
Deanship
The Dean
Dean's Word
Curriculum Vitae
Contact the Dean
Vision and Mission
Organizational Structure
Vice- Deanship
Vice- Dean
KAU Graduate Studies
Research Services & Courses
Research Services Unit
Important Research for Society
Deanship's Services
FAQs
Research
Staff Directory
Files
Favorite Websites
Deanship Access Map
Graduate Studies Awards
Deanship's Staff
Staff Directory
Files
Researches
Contact us
عربي
English
About
Admission
Academic
Research and Innovations
University Life
E-Services
Search
Deanship of Graduate Studies
Document Details
Document Type
:
Thesis
Document Title
:
Enhancement of biologically active exopolysaccharides production by Bacillus firmicutes
تعزيز إنتاج السكريات العديدة الخارجية النشطة حيوياً بواسطة باسيلوس فيرميكوتس
Subject
:
Faculty of Science
Document Language
:
Arabic
Abstract
:
Our worked deal with the production of exopolysaccharide by bacteria from dates seed powder as solo carbon source. A pure bacterial colony was isolated from hot spring and identified by phylogenetic as B. firmicutes. Bacterial strain, B. firmicutes was capable of producing EPS using very cheap carbon ago seeds pits as solo carbon source. Important parameters were studied to enhance exopolysaccharide production. Maximum total sugars (exopolysaccharide) were reached to 0.87 mM at 20 g/L agwa dates seed (ADS). The maximum production was found to be 3.46 mM by addition of peptone as main source of nitrogen with concentration 1.5 g/L. The optimal parameter values were temperature 37°C, pH 6, incubation time 72h and inoculum concentration 1 ml. The crude exopolysaccharide was purified by removing the cells, then the protein, dialysis the exopolysaccharide solution then precipitation by ethanol. The purified exopolysaccharide about (0.5 g) was hydrolyzed and analyzed by HPLC. The components of monosaccharides were glucouronic acid, fructose and glucose with a molar ratio of 2.2: 1.3: 1.0 respectively. Furthermore, it also proved that B. firmicutes exopolysaccharide showed significant activity as antioxidant as detected by DPPH and antiproliferative activity on Ehrlich tumor.
Supervisor
:
Prof. Dr. Youssri M. Ahmed Soliman
Thesis Type
:
Master Thesis
Publishing Year
:
1440 AH
2019 AD
Co-Supervisor
:
Dr. Wesam H Abdulaal
Added Date
:
Wednesday, July 24, 2019
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
راكان هشام يوسف
Yousef, Rakan Hisham
Researcher
Master
Files
File Name
Type
Description
44802.pdf
pdf
Back To Researches Page