|
Document Type |
: |
Thesis |
|
Document Title |
: |
IN VIVO EVALUATION OF THE ANTICANCER ACTIVITY OF DOCETAXEL-LOADED NANOPARTICLES التقييم الحيوي للنشاط المضاد للسرطان للدوسيتاكسال المحمل على جسيمات متناهية الصغر |
|
Subject |
: |
Faculty of Sciences |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Docetaxel (Doc), an antitumor drug used to treat various types of cancers, has certain limitations in clinical use due to its side effects. The major objective of this study was to load Doc in nanoemulsion based on garlic oil (Doc-NEGO) and to evaluate the efficacy and cytotoxicity of the resulted formula in female Swiss Albino mice inoculated with Ehrlich ascites carcinoma (EAC) cells. One hundred mice were divided into five groups (n =20). Groups I and II served as G(-) and G(+), respectively, while groups III-V represented the EAC-mice administered every two day via oral gavage with 3 doses of 24 mg of Doc/kg of the mice weight of Doc loaded in 0.2mL of NEGO (G(NDoc)), 0.2mL of NEGO (G(NE)) and 24 mg of Doc/kg of the mice weight of Doc loaded in 0.2mL of distilled water (G(WDoc)), respectively. The antitumor activity was evaluated by detecting the efficacy of the administered drug through measuring the tumor volume, body weight and lactate dehydrogenase activity (LDH) in the ascetic fluids. The hepatotoxicity, cardiotoxicity and nephrotoxicity were identified by determining the serum enzymes, analyzing the reactive oxidative stress and studying the histological changes of the liver, heart and kidney tissues. The z-average diameters of the nanoparticles of NEGO and Doc-NEGO determined by the zetasizer were (63.19 ± 1.85 nm) and (110.43± 14.37 nm), respectively. Treating the EAC-bearing mice with the Doc-NEGO has improved the mean survival time (MST) of the mice (27.7 ± 11.63 days) relative to the MST of G(WDoc) (23.1 ± 1.52 days). Compared to G(WDoc), the catalase and glutathione peroxidase activities of G(NDoc) were enhanced in the hepatocytes and cardiomyocytes, respectively. In conclusion, NEGO holds a great potential as a nanocarrier for the Doc in improving its efficacy and eliminating its cardiotoxicity. |
|
Supervisor |
: |
prof. Mayson Husni Alkhatib |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1439 AH
2018 AD |
|
Co-Supervisor |
: |
Prof. Wadiah Salah Backer |
|
Added Date |
: |
Monday, March 26, 2018 |
|
Researchers
| بشائر محمد صديق | siddiq, Bashair Mohammed | Researcher | Master | |
|