Document Details

Document Type : Thesis 
Document Title :
Molecular studies on some cell cycle genes of Saccharomyces cerevisiae treated with Rhazya stricta and Curcuma longa extracts
دراسات جزيئية على بعض جينات دورة الخلية في خميرة الخباز المعاملة بنباتات الحرمل والكركم
 
Subject : Faculty of Sciences > biological sciences department 
Document Language : Arabic 
Abstract : The aim of the present study is to determine the genetic responses of Saccharomyces cerevisiae treated with the aqueous extract of Rhazya stricta leaves (mutagenic) that commonly used in folk medicine in Saudi Arabia and the aqueous extract of Curcuma longa powder (antimutagenic) as well as their mixture for 30 and 60 min for each treatment. Genetic toxicity of aqueous extract of R. stricta revealed 11 auxotrophic mutants with reduction in survival percentages by increasing the duration of treatments. No mutants were obtained with aqueous extracts of C. longa and R x C mixture. SDS-PAGE analysis of S. cerevisiae total proteins characterized each mutant with distinctive bands. Mutagenic effects of R. stricta mutants were determined by the presence of protein bands while they were absent in the control. The results of protein and RAPD analyses using two primers; OPY-02 and OPA-11 indicated that some distinctive protein bands and amplified fragments that affected by R. stricta were recovered by Curcuma and R. stricta mutagenic effects were no more existed in the mixture. Two designed primers were detected the cell cycles genes CDC5 and CDC9. Analysis of nucleotide sequences revealed the effects of Razya extract in the three genes. A total of 9 base-pair substitutions were obtained using Razya treatment versus 12 for Curcuma, whereas 5 nucleotides were changed by Razya in CDC5 vs. 8 by Curcuma, 4 by Razya vs. 4 by Curcuma in CDC9. Restoration of the nucleotide base-pair in the three genes was high in the mixture comparing with the treatment of toxic Razya; 26 vs. 9. The antimutagenic Curcuma in the mixture showed high ability to recover and restore the three cell cycle genes in S.cerevisiae to their normal functions and growing the cells normally until completing their cell cycles after treatment of the Razya toxic species. Therefore, the present study recommend to adding anticancer Curcuma longa to Rhazya stricta in a mixture to use as traditional folk medicine in Saudi Arabia and other countries. 
Supervisor : Prof . Dr . Nada Hassan tawate 
Thesis Type : Master Thesis 
Publishing Year : 1435 AH
2014 AD 
Co-Supervisor : Dr. Nariman Abdel-Mongy Hassan Aly 
Added Date : Tuesday, August 19, 2014 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
غدي اسامة العباسيAlabbasi, Ghadi OsamaResearcherMaster 

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