Document Details

Document Type : Article In Journal 
Document Title :
Characterization of Mucor racemosus lipase with potential application for the treatment of cellulite
Characterization of Mucor racemosus lipase with potential application for the treatment of cellulite
 
Subject : Biochemistry 
Document Language : English 
Abstract : An extracellular lipase LII from Mucor racemosus was purified by anion exchange and gel filtration chromatography using DEAE-Sepharose and Sephacryl S-200 columns, respectively. A molecular weight of 20 kDa was estimated for M. racemosus LII by Sephacryl S-200 column and SDS-PAGE. LII had an acidic pI at 4.8. Vmax and Kmfor LII were estimated to be 55.5 μmol oleic acid/min/ml and 2% olive oil, respectively. The highest lipolytic activity was detected with linseed oil as the substrate. The optimal pH and temperature for LII was 5.0 and 40 °C, respectively. The enzyme was stable up to 70 °C. The lipase activity was strongly enhanced by Cu2+, Al3+ and Fe3+, and slightly enhanced by Mg2+, Ni2+ and Mn2+, whereas Ca2+, Co2+, K+ and Hg2+ showed no effect. Pb2+ only caused a partial inhibition of the enzyme. The residual lipase activity in carbopol 934 base gel was 385% after storage at 4 °C for 420 days. The characteristic properties of M. racemsus lipase formulated in the carbopol 934 gel was utilized for topical treatment of cellulite, and the results were positive for reducing thigh circumference. 
ISSN : 1359-5113 
Journal Name : Process Biochemistry 
Volume : 46 
Issue Number : 3 
Publishing Year : 1432 AH
2011 AD 
Article Type : Article 
Added Date : Saturday, February 18, 2012 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
صالح أحمد محمدMohamed, Saleh AhmedResearcherDoctoratesaleh38@hotmail.com
Heidi M Abdel-MageedAbdel-Mageed, Heidi MResearcherDoctorate 
Saadia A TayelTayel, Saadia AResearcherDoctorate 
Mohamed A El-NabrawiEl-Nabrawi, Mohamed AResearcherDoctorate 
Afaf S FahmyFahmy, Afaf SResearcherDoctorate 

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