Main Page
Dean's Introduction
Org Structure
About RACI
Deans of the Institute
Our Partners
Our Mission and distinctiveness
Quality Policy
Members of the Advisory Committee
More about RACI's administration
the Institute's objectives
our capabilities
more of Capacity Institute
decisions & generalizations
List of Sc.
Int'l Scientific Chairs
Scientific Chairs
Quotes on KAU’s Scientific Chairs
history Scientific Chairs
Publications and Research Institute consultations
models and success stories
Sponsors’ Guide
Researchers’ Guide
Scientific publications
Sponsored chairs
Institute Activities
final championship Institute
accreditation EFQM
Ramadan breakfast
Our Services
Our Services And Capabilities
Scientific Research Chairs
Quotes on KAU’s Scientific Chairs
History Scientific Chairs
Publications and Research Institute consultations
Models And Success Stories
Sponsors’ Guide
Researchers’ Guide
Consulting
Quotes on RACI Consultation Services
Our Partners
Consultation Archive 1419
Consultation Archive 1420
Consultation Archive 1421
Consultation Archive 1422
Consultation Archive 1423
Consultation Archive 1424
Consultation Archive 1424
Consultation Archive 1425
Consultation Archive 1426
Consultation Archive 1427
Consultation Archive 1428
Consultation Archive 1429
Contract research and studies
Our Partners
research and studies Contractual - Medical
research and studies Contractual - Engineering
Economic Projects
Quotes on RACI research and studies Services
Supervision of the educational and academic
Educational Supervision
Our Partners
Curriculum Design
Academic Training
Curriculum Assessment
Scientific services
Exhibition Service's continued scientific
My Previous Scientific Services
Our capabilities
General Statistics
Academic Supervision
Classification of Scientific Chairs
Classification of institute activities
Institute Activities According The Sector
Classification of Contract Research
classification of dedicated consultations
Academic Supervision
Files
RACI Map
Research
PhotoAlbum
Contact Us
Favorite Links
Latest News
عربي
English
About
Admission
Academic
Research and Innovations
University Life
E-Services
Search
Research And Consulting Institute
Document Details
Document Type
:
Article In Journal
Document Title
:
Ultrastructural immuno gold study on the various cell types of cultured pancreatic islets of adult rats
دراسة الذهب التركيبية المناعية على مختلف أنواع الخلايا من الجزر البنكرياسية مثقف من الفئران الكبار
Subject
:
Ultrastructural immuno gold study on the various cell types of cultured pancreatic islets of adult rats
Document Language
:
English
Abstract
:
Whereas several reports describing the ultrastructure of the intact pancreatic islets have been recorded, published experience with the ultrastructural integrity of the cultured pancreatic islets is limited. The present study was, therefore, undertaken to provide an ultrastructure identification of the different cells in the cultured islets of the adult rat pancreas, after marking their secretory granules with gold particles. Pancreatic islets were isolated from adult male Wistar rats by the intraductal perfusion of collagenase technique. The islets were cultured in RPMI-1640 medium for 3 days and processed for preparation of ultrathin sections. The sections were stained with the indirect immunogold technique for insulin, glucagon, somatostatin, and pancreatic polypeptide. Ultrastructural examination of the cultured islets clearly identified the presence of B, A, D and PP-cells, as indicated by the numerous gold particles concentrated predominantly over the secretory granules. The secretory granules of the various cell types of the cultured islets demonstrated several similarities as well as differences from the recorded results of the corresponding secretory granules of the intact islets. The differences probably reflect a deviation in the underlying mechanisms of synthesis, maturation and secretion of the different secretory products of the cells in the cultured islets as they adapt to the in vitro environment.
ISSN
:
0015-5659
Journal Name
:
Folia Morphol
Volume
:
59
Issue Number
:
4
Publishing Year
:
1420 AH
2000 AD
Article Type
:
Article
Added Date
:
Saturday, February 19, 2011
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
مصطفى النجار
alnajar, mustafa
Investigator
Doctorate
Files
File Name
Type
Description
29053.pdf
pdf
Back To Researches Page