Main Page
عربي
English
About
Admission
Academic
Research and Innovations
University Life
E-Services
Search
Faculty of Science and Arts - Khulais
Document Details
Document Type
:
Article In Journal
Document Title
:
PURIFICATION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC
تنقية و تعيين سمية طحلب الأسولاتوريا بواسطة جهاز الفصل الكروماتوجرافى عالى الحساسية
Subject
:
toxicity
Document Language
:
English
Abstract
:
Aqueous extract of toxic Oscillatoria collected from the Solar Lake was purified using ultrafiltration technique and gel filtration column chromatography. Mouse bioassay was used to trace the toxicity during purification steps. Dose response curve of the purified material was compared to that of the crude extract. A reverse phase HPLC technique (isocratic elution with 2% acelonitrile aqueous solution adjusted lo pH 2.5 with phosphoric acid and UV detection at 254 nm) was suggested to detect the toxic compound in the crude cyanobacterial extracts of the Solar Lake. Toxic and non toxic fractions of the gel filtration were used to identify the responsible peak for toxicity by HPLC.
ISSN
:
2341-456
Journal Name
:
J. Egypt. Soc. Toxicol
Volume
:
201
Issue Number
:
1
Publishing Year
:
1420 AH
1998 AD
Article Type
:
Article
Added Date
:
Saturday, January 8, 2011
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
محمد نصر الدين محمد جمعة
Gomaa, Mohamed Nasr Eldin mohamed
Investigator
Doctorate
mngomaa@gmail.com
Files
File Name
Type
Description
28506.pdf
pdf
Back To Researches Page