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Deanship of Scientific Research
Document Details
Document Type
:
Project
Document Title
:
Comparison of Microscopy and PCR for Detection and Identification of Cryptosporidium Species in Human Fecal Specimens in Jeddah
مقارنة بين الفحص المجهري واختبار تفاعلات البلمرة التسلسلي لتشخيص طفيلي الكريبتوسبوريديوم في عينات براز بمدينة جدة
Subject
:
Comparison of Microscopy and PCR for Detection and Identification of Cryptosporidium Species in Human Fecal Specimens in Jeddah
Document Language
:
Arabic
Abstract
:
Cryptosporidium is an opportunistic intestinal protozoa that infects humans causing chronic watery diarrhea and could be life threatening in immunocompromised patients and children. Water out-breaks of cryptosporidiosis have been widely reported. Microscopic examination of acid fast stained smears is considered as the most common routinely technique for diagnosis of Cryptosporidium oocysts. In a previous unpublished study with the above stain, Wakid found that 99.2% of the examined samples were positive for oocysts. This result needs confirmation as if oocysts of other acid fast organisms could show same size and reaction with the stain. Polymerase chain reaction (PCR) is highly sensitive and specific for detection and identification of organisms. In this study we will diagnose Cryptosporidium in fecal samples from patients in Jeddah by different technique of PCR and compare it with the acid fast technique.
Publishing Year
:
1428 AH
2007 AD
Sponsor Name
:
King Abdulaziz University
Sponsorship Year
:
1428 AH
2007 AD
Added Date
:
Tuesday, June 29, 2010
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
ماجد حمدي واكد
Wakid, Majed Hamdi
Investigator
Doctorate
Files
File Name
Type
Description
27189.docx
docx
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