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Faculty of Sciences
Document Details
Document Type
:
Article In Journal
Document Title
:
Properties of the V-type ATPase from the excretory system of the usherhopper, Poekilocerus bufonius
Properties of the V-type ATPase from the excretory system of the usherhopper, Poekilocerus bufonius
Document Language
:
Arabic
Abstract
:
The bafilomycin A, and N-ethylmaleimide (NEM)-sensitive (V-type) ATPase was partially purified from the apical membrane-rich fractions of excretory system (Malpighian tubules and hind gut) of P. bufonius. Enzymatic activity was inhibited by bafilomycin A(1) (IC50 = 1.3 nM) and NEM (IC50 = 10.1 muM). The V-type ATPase activity is confined to the apical membrane fraction, while the activity of Na+/K+ -ATPase forms the major part of the basal membrane fraction. The optimal pH required for maximal activity of V-type ATPase was pH 7.5. The effect of 30 mM of various salts on ATPase activity was investigated. NaCl and KCl caused increases of 175% and 184%, respectively. Other chloride salts also caused an increase in activity in the following ascending order: RbCl, LiCI, choline Cl, NaCI, KCl and tris-HCl. The activity of V-type ATPase was stimulated by a variety of different anions and cations, and HCO3- was found to be the most potent cationic activator of ATPase activity. The present results show that the properties of V-type ATPase of P. bufonius are similar to those reported for other insect tissues. (C) 2002 Elsevier Science Ltd. All rights reserved.
Journal Name
:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume
:
32
Issue Number
:
9
Publishing Year
:
2002 AH
Added Date
:
Monday, June 23, 2008
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
د. زراق عيسى علي الفيفي
Z. I. A. Al-Fifi
Researcher
z_alfifi@hotmail.com
أ.د. علي بن احمد بن سالم الرباعي
Al-Robai A
Researcher
alrobai@hotmail.com
سمير محمد خوجه
Khoja SM
Researcher
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